Coding

Part:BBa_M36067

Designed by: Rahul Sastry, Anjan Katta   Group: Stanford BIOE44 - S11   (2013-06-10)


fnr-gal4 hybrid

This part codes for the fnr-gal4 protein. FNR is a transcriptional activator in E. coli that is sensitive to oxygen levels. The protein is has an iron-sulfur code that, when bound to oxygen, undergoes a conformational change that reduces its ability to activate transcription. This particular coding sequence includes a nuclear localization sequence at the N-terminus (for application in eukaryotes), a gal4 activation domain (also at the N-terminus), and a stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 970
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 970
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 970
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 970
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None